Fig. 4

The histone deacetylase inhibitor TSA potently relieves silencing of unintegrated HIV-1 DNAs in HeLa cells but only weakly in lymphoid K562 cells. Cells were infected with IN-D64A in the presence of TSA or DMSO. Flow cytometry analyses were performed 24 h after virus application. A Representative flow cytometry plots are shown of infected HeLa and K562 cells treated with either TSA or DMSO. B Number of ZsGreen-positive cells (left panel) and the MFI of ZsGreen-positive cells are shown (right panel). Error bars indicate mean ± SD. Mock represents uninfected control. Each dot represents an independent replicate