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Fig. 3 | Retrovirology

Fig. 3

From: Two lymphoid cell lines potently silence unintegrated HIV-1 DNAs

Fig. 3

Total virus DNA levels after infection with IN-D64A and IN-wt virus are comparable in all cell lines. DNA was isolated from K562 cells (A), Jurkat cells (B) and HeLa cells (C) and the total viral DNA levels were determined by qPCR using ZsGreen-specific primers (left panel). 2-LTR circle levels were measured utilizing 2-LTR-specific primers (right panel). Levels are shown relative to GAPDH. Error bars indicate mean ± SD. Mock represents uninfected control. Each dot represents an independent replicate. All experiments were performed a minimum three times, and in many cases six times as indicated by the dots. The cases with no detected signal in the PCR runs lie below the X-axis in log scale data presentations (e.g. the Mock samples) and are left blank

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