Skip to main content
Fig. 7 | Retrovirology

Fig. 7

From: Innate immune regulation in HIV latency models

Fig. 7

Single cell RNA sequencing analysis of ISG induction in HIV-suppressed, primary CD4 + T cells CD4 + T cells (donors #1 & 2) were mock-infected (media) or HIV-infected (NanoLuc HIV MOI 2.0) for 24 h, then suppressed with ART for 7 days. At day 8 cells were left untreated or were stimulated with 100 IU/ml IFNβ for 8 h, then analyzed by scRNA-seq (see schematic in Fig. 5b). a Differential expression (DE) analysis of bulk populations of IFN-treated mock vs. untreated mock cultures (black circles), and IFN-treated HIV-infected vs. untreated HIV-infected cultures (red circles). We identified 116 ISGs that were significantly differentially expressed in any IFN-treated sample relative to corresponding untreated control (Log2 fold change > 1.2, p < 0.05). b–d Log2 fold change expression of 116 ISGs in each vRNA subset from IFN-treated, HIV-infected populations (vRNAhi, vRNAlo, or vRNA-) relative to IFN-treated, mock-infected cells (see Additional file 3: Table S2). For all graphs above, dotted lines represent control to which graphed samples are normalized (a IFN-untreated cells; b–d, y axis: untreated mock-infected cells; b–d, x axis: IFN-treated mock-infected cells). e Log2 fold change gene expression in HIV-infected relative to similarly treated mock-infected cells, for select ISGs from graphs 7b–d

Back to article page