No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

Retrovirology

Table 2 Summary of anti-BLV antibody detection in human serum by ELISA

^aBLV gp51 and p24 ELISAs in BLV gp51 and p24 antigens coated plates was performed using 50-fold-diluted human serum specimens as primary antibody following either HRP-conjugated goat anti-human IgG or HRP-conjugated goat anti-human IgM as secondary antibody. NT indicates not tested
^bBLV gp51 and p24 ELISAs was performed using two BLV-infected cattle and one cattle with no BLV infection, as positive and negative controls, respectively, followed by HRP-conjugated anti-bovine IgG, as second antibody to check the validity of the experiment procedure
^cHTLV I/II antibody ELISA was performed using human HTLV I/II positive antibody and negative antibody, followed by HRP-conjugated goat anti-human IgG and IgM in replacement with the second antibody in the kit to validate whether anti-human second antibodies HRP-conjugated goat anti-human IgG and IgM used in our study are actively working or not

ISSN: 1742-4690