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Table 2 Summary of anti-BLV antibody detection in human serum by ELISA

From: No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan

2nd Antibody Human sera specimens Control experiment
Bovine sera Human sera
BLV gp51a ELISA (N = 97) BLV p24a ELISA (N = 97) BLV gp51 ELISAb BLV p24 ELISAb HTLV-I/II ELISAc
BLV (+) (N = 2) BLV (−) (N = 1) BLV (+) (N = 2) BLV (−) (N = 1) HTLV (+) HTLV (−)
Goat anti-human IgG (HRP) 0/97 0/97 0/2 0/1 0/2 0/1 2/2 0/2
Goat anti-human IgM (HRP) 0/97 0/97 0/2 0/1 0/2 0/1 2/2 0/2
Rabbit anti-bovine IgG (HRP) NT NT 2/2 0/1 2/2 0/1 0/2 0/2
  1. aBLV gp51 and p24 ELISAs in BLV gp51 and p24 antigens coated plates was performed using 50-fold-diluted human serum specimens as primary antibody following either HRP-conjugated goat anti-human IgG or HRP-conjugated goat anti-human IgM as secondary antibody. NT indicates not tested
  2. bBLV gp51 and p24 ELISAs was performed using two BLV-infected cattle and one cattle with no BLV infection, as positive and negative controls, respectively, followed by HRP-conjugated anti-bovine IgG, as second antibody to check the validity of the experiment procedure
  3. cHTLV I/II antibody ELISA was performed using human HTLV I/II positive antibody and negative antibody, followed by HRP-conjugated goat anti-human IgG and IgM in replacement with the second antibody in the kit to validate whether anti-human second antibodies HRP-conjugated goat anti-human IgG and IgM used in our study are actively working or not