YTHDC1 regulates distinct post-integration steps of HIV-1 replication and is important for viral infectivity

Retrovirology

Table 2 qPCR primers

Gene	Forward	Reverse	Refs.
Total viral RNA	TTGCTCAATGCCACAGCCAT	TTTGACCACTTGCCACCCAT	[34, 52]
US	TTCTTCAGAGCAGACCAGAGC	GCTGCCAAAGAGTGATCTGA	[52]
IS	GGCGGCGACTGGAAGAAGC	CTATGATTACTATGGACCACAC	[52]
MS	CTGAGCCTGGGAGCTCTCTGGC	CCGCAGATCGTCCCAGATAAG	[53]
Nef2	AGGGGCGGCGACTGGAAGA	GATTGGGAGGTGGGTTGCTTTG	[34, 52]
Rev2	AGGGGCGGCGACTGCCTTA	GATTGGGAGGTGGGTTGCTTTG	[34, 52]
Tat1	AGGGGCGGCGACTGAATTGGGT	GATTGGGAGGTGGGTTGCTTTG	[52]
Env1	AGGGGCGGCGACTGGAAGA	CTATGATTACTATGGACCACAC	[34, 52]
Vpr3	GCGGCGACTGAATCTGCTAT	GCTGCTAGTGCCAAGTACTG	[34, 52]
Vif2	GCGGCGACTGGGACAGCAGAG	GGCACTACTTTTATGTCACT	[34, 52]
preGAPDH	CCACCAACTGCTTAGCACC	CTCCCCACCTTGAAAGGAAAT
GAPDH	TGCACCACCAACTGCTTAGC	GCATGGACTGTGGTCATGAG
YTHDF1	CAAGCACACAACCTCCATCTTCG	GTAAGAAACTGGTTCGCCCTCAT
YTHDF2	TAGCCAGCTACAAGCACACCAC	CAACCGTTGCTGCAGTCTGTGT
YTHDF3	CGGACAGTGATGCCTACAACAG	TCTGTGAGGTGCGAGGGACTAA

ISSN: 1742-4690