Fig. 6

YTHDC1 depletion affects the incorporation of mature Env into viral particles. A HIV-1 viral proteins Env and Gag in cells supernatants were analyzed by western blot. Equal amounts of CAp24 were loaded per lane to assess Env content per fixed amounts of viral particles. B Env signals were quantified and values obtained were used to calculate the Env processing efficiency (ratio of the level of processed SUgp120 relative to the level of total Env (Env gp160 + SUgp120)). Data are presented as mean ± S.D. (n = 3). P values were calculated using unpaired t-test (**p < 0.01). C Infectivity of virions was measured by HeLa TZM-bl reporter assay and normalized to the quantity of CAp24 in the supernatants. Values are presented as means ± SD (n = 6). P values were calculated using one sample t-test (*p < 0.05, ***p < 0.001)