Fig. 4

YTHDC1 interacts with the 3 classes of HIV-1 mRNAs in a METTL3-dependent manner. A Western blot showing that endogenous YTHDC1 was specifically immunoprecipitated from HeLa/LAV cells. IgGs were used as control immunoprecipitation. B HeLa/LAV cells were transfected with control (Ctrl) or METTL3 and RBM15-targeting siRNA and 3 days later, knock down of METTL3 and RBM15 was confirmed by western blot. C YTHDC1 was immunoprecipitated from HeLa/LAV cells transfected in (B) and levels of YTHDC1-associated US, IS and MS viral RNAs were quantified by RT-qPCR. Data are presented as mean ± S.D. (n = 4). P values were calculated using paired t-test (*p < 0.05, **p < 0.01). D Same as (C), except the relative abundance of MS RNAs Tat1 and Rev2 and IS RNA Env1 was monitored by RT-qPCR. Results are expressed as percentage of input. Data are presented as mean ± S.D. (n = 3). P values were calculated using paired t-test (*p < 0.05, **p < 0.01)