Fig. 2

Downregulation of YTHDC1 increases US and IS viral RNAs without affecting the abundance of MS transcripts. A HeLa/LAV cells were transfected with a control (Ctrl) or YTHDC1-targeting siRNA (DC1-1). 3 days later, YTHDC1 knock down was confirmed by western blot. B The relative abundance of Total, US, IS and MS HIV-1 RNAs was monitored by RT-qPCR. C Levels of US, IS and MS RNAs from (B) were normalized to the level of Total viral RNA. D The relative abundance of IS RNAs, Env1, Vpr3 and Vif2, an MS RNAs Tat1 and Nef2 was monitored by RT-qPCR. Results are expressed in fold change over the control siRNA (Ctrl). Data are presented as mean ± S.D. (n = 4). P values were calculated using one sample t-test (*p < 0.05, **p < 0.001, ***p < 0.0001)