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Fig. 1 | Retrovirology

Fig. 1

From: YTHDC1 regulates distinct post-integration steps of HIV-1 replication and is important for viral infectivity

Fig. 1

Depletion of m6A writer complex subunits increase HIV-1 mRNA levels in infected cells. A HeLa cells were transfected with a control (Ctrl) or METTL3, WTAP, VIRMA and RBM15-targeting siRNAs, as indicated. Cellular proteins knockdown was confirmed by western blot analysis of cell lysates transfected with indicated siRNAs. B 3 days after siRNA (targeting RBM15) or 4 days after siRNA transfection (targeting METTL3, WTAP and VIRMA), cells were infected with a single round VSVg-pseudotyped HIV-1 virus. 24 h.p.i., the relative abundance of unspliced (US), incompletely spliced (IS) and multiply spliced (MS) HIV-1 RNAs was monitored by RT-qPCR. Results are expressed in fold change over the control siRNA (Ctrl). Data are presented as mean ± S.D. (n = 3). P values were calculated using one sample t-test (*p < 0.05, **p < 0.001)

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