Fig. 4

Effect of different agents to activate the inducible peripheral HIV-1 reservoir. Memory CD4⁺ T cells from Ugandan (n = 4) and U.S. (n = 5) patients were analyzed with the EDITS assay using, in addition to the standard 10 µg/ml of Concanavalin, a combination of IL-15 50 ng/ml plus SAHA 500 nM, IL-15 50 ng/ml plus GSK343 500 nM, or IL-15 50 ng/ml plus UNC638 500 nM. Unpaired t test was used to compare the reservoir size (number of cell equivalents per one million cells) induced by Concanavalin A vs the combination of agents to induce gene expression. Median cell equivalents/million cells and interquartile range are depicted. n.s., not significant