Table 2 Primers
From: HIV-2/SIV Vpx antagonises NF-κB activation by targeting p65
Target | Sequence |
|---|---|
GAPDH | Fwd 5′‐GGGAAACTGTGGCGTGAT‐3′ Rev 5′‐GGAGGAGTGGGTGTCGCTGTT‐3′ |
CXCL-10 | Fwd 5′‐TGGCATTCAAGGAGTACCTC‐3′ Rev 5′‐TTGTAGCAATGATCTCAACACG‐3′ |
TNF-α | Fwd 5′-AGCCTCTTCTCCTTCCTGATCGTG-3′ Rev 5′-GGCTGATTAGAGAGAGGTCCCTGG-3′ |
IFIT-1 | Fwd 5′-CCTCCTTGGGTTCGTCTACA-3′ Rev 5′-GGCTGATATCTGGGTGCCTA-3′ |
IL-8 | Fwd 5′-GAGAGTGATTGAGAGTGGACCAC-3′ Rev 5′-CACAACCCTCTGCACCCAGTTT-3′ |
Vpx E15A E16A | Fwd 5′-CAATAGCGGCGCCGCAACCATCGAAG-3′ Rev 5′-CCAGGAGGGATTCTCTCTC-3′ |
Vpx Q47A V48A | Fwd 5′-GCTGATCTTCGCCGCGTGGCAGAGAAGCTGGG-3′ Rev 5′-TCGCGAGGCAGATGGTTC-3′ |
Vpx R51A S52A | Fwd 5′-GCTAATTTTCCAGGTTTGGCAAGCGGCCTGGGAATACTGG-3′ Rev 5′-CCAGTATTCCCAGGCCGCTTGCCAAACCTGGAAAATTAGC-3′ |
Vpx Q76R | Fwd 5′-GTGCCTGATCAGGAAAGCCCTGTTC-3′ Rev 5′-AGGTATCTGTACTTGGTG-3′ |