Fig. 4
From: HIV-2/SIV Vpx antagonises NF-κB activation by targeting p65
Vpx interacts with p65. A Immunoblot from co-immunoprecipitation assay performed from HEK293T cells co-transfected with FLAG-SIVmac Vpx and HA-tagged p50, p65, p100, IKKβ or an EV control. Whole cell lysates (WCL) were probed with FLAG, HA and tubulin antibodies and immunoprecipitates (IP) were probed for FLAG and HA following immunoprecipitation with anti-HA beads. B Immunoblot from co-immunoprecipitation assay performed from HEK293T cells co-transfected with HA-p65 and FLAG-SIVmac Vpx or an EV control. WCL and IP samples that had been incubated with anti-FLAG beads were probed with HA and FLAG antibodies. Data are from a representative experiment repeated 4 times