Table 1 Primers for PCR amplification and Quantitative real-time PCR primers

qPCR-actin-F

5′-CACGATGGAGGGGCCGGACTCATC-3′

qPCR-actin-R

5′-TAAAGACCTCTATGCCAACACAGT-3′

qPCR-gag-F

5'-AATAGCGGGCGGGGACGACA-3'

qPCR-gag-R

5'-ATTGCCACGCACCCCAGAGC-3'

qPCR-tas-F

5'-GGAACAATCAGATACTGACCCT-3'

qPCR-tas-R

5'-CCAACTTCAGGATCCCATCTT-3'

ChIP-GAPDH-pro-F

5′-GAAGGTGAAGGTCGGAGTCA-3′

ChIP-GAPDH-pro-R

5′-CCCATACGACTGCAAAGACC-3′

ChIP-LTR-U3-1-F (52–187)

5′-GGGAAGGAAGTGAAGAAC-3′

ChIP-LTR-U3-1-R (52–187)

5′-TTGGATGTCAGAGGGAGT-3′

ChIP-LTR-U3-2-F(151–166)

5′-ACTCCCTCTGACATCC-3′

ChIP-LTR-U3-2-R(353–368)

5′-TTTTCGGTGTCTGTCA-3′

ChIP-LTR-U3-3-F(355–370)

5′-AAGCCACAGACAGTAA-3′

ChIP-LTR-U33-3-R(574–589)

5′-TGCATCCCACTGTTCT-3′

ChIP-LTR-U3-4-F (573–588)

5′-CACGTAGGGTGACAAG-3′

ChIP-LTR-U3-4-R (584–800)

5′-GAGAAGTGATGAGCGAC-3′

ChIP-LTR-R-F (831–955)

5′-GCTCTTCACTACTCGCTG

ChIP-LTR-R-R (831–955)

5′-GCAATCACCCTTACAATC

ChIP-LTR-U5PBS-F (1072–1167)

5′-CTTAAATGATGTAACTCCT-3′

ChIP-LTR-U5PBS-R (1072–1167)

5′-TACAAATAAACCCGACTT-3′

ChIP-IP-1-F (9019–9108)

5′-CTGGACTTTAAAAGGCCACT-3′

ChIP-IP-1-R (9019–9108)

5′-AACCAAATGTGGTAATCT-3′

ChIP-IP-2-F (9013–9196)

5′-TTTGGTTGGAATTATTGC-3′

ChIP-IP-2-R (9013–9196)

5′-AGCTTTTGCTCTTTCAAT-3′

pSuper-shTrim28-F (target sequence)

5′-GATCCCCGCGTCCTGGCACTAACTCATTCAAG

AGATGAGTTAGTGCCAGGACGCTTTTTA-3′

pSuper-shTrim28-R (target sequence)

5′-GATCCCCGCATGAACCCCTTGTGCTGTTCAAG

AGACAGCACAAGGGGTTCATGCTTTTTA-3′

LTR-U3-F/Mlu I (pGL3-PFV-LTR-U3-luc)

5′-ATACGCGTTGTGGTGGAATGCC-3′

LTR-U3-R/Bgl II (pGL3-PFV-LTR-U3-luc)

5′-GGAAGATCTCCCGTACAAT-3′

LTR-U3-R-R/Bgl II (pGL3-PFV-LTR-U3R-luc)

5′-GGAAGATCTAGGTTCTCGAAT-3′

LTR-U3-R-U5-R/Bgl II (pGL3-PFV-LTR-U3RU5-luc)

5′-GGAAGATCTATTGTCATGGAA-3′

LTR-PBS-R/Bgl II (pGL3-PFV-LTR-U3RU5pbs-luc)

5′-GAAGATCTGCCCCACGTTG-3′