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Fig. 1 | Retrovirology

Fig. 1

From: Trim28 acts as restriction factor of prototype foamy virus replication by modulating H3K9me3 marks and destabilizing the viral transactivator Tas

Fig. 1

Trim28 inhibits prototype foamy virus replication. a The relative viral load in the presence or absence of overexpressed Trim28 was analyzed in PFV indicator cell line using the FAL assay. RL-TK (5 μg) was transfected as an internal control. b HT1080 and HEK293T cells were transfected with pCMV-Flag-Trim28 or pCMV-Flag (as an empty control) for 24 h. After transfection, cells were infected with PFV (MOI = 0.1) for 48 h. The expression of PFV Gag and Tas were detected by Western blot. c Specific shRNAs was used to knockdown Trim28 and the shControl was used as negative control. The viral proteins Gag and Tas were detected by Western blot. Quantitation analysis of Gag and Tas intensity from the western blotting using Quantity one software (Bio-Rad). d Relative mRNA expression (normalized to β-actin) of the viral structural gene gag and of all genes encompassing the tas region in the PFV-infected HEK293T or HT1080 cells that were transfected with pCMV-Flag-Trim28 or pCMV-Flag (as an empty control) for 24 h. e Relative mRNA expression (normalized to β-actin) of the viral structural gene gag and of all genes encompassing the tas region in the PFV-infected HEK293T or HT1080 cells that were transfected with shTrim28 or shControl were assessed by qPCR. All the data are representative of three independent experiments with triplicate samples. (paired t-test; *p < 0.05, **p < 0.01, ***p < 0.001)

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