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Table 1 Antibody sources and conditions

From: Residues T48 and A49 in HIV-1 NL4-3 Nef are responsible for the counteraction of autophagy initiation, which prevents the ubiquitin-dependent degradation of Gag through autophagosomes

Protein or tag Antibody Dilution Source
ACTB/β-actin Mouse monoclonal (C4) to ACTB/β-actin 1:1000 Sigma-Aldrich, MAB1501
BCL2 Mouse monoclonal (124) to BCL2 1:1000 Cell Signaling Technology, 15071S
BECN1 Rabbit monoclonal (D40C5) to BECN1 1:1000 Cell Signaling Technology, 3495S
CD4 Mouse monoclonal (RPA-T4) to CD4 (FITC-conjugated) 1:200 BD biosciences, 561005
GFP Mouse monoclonal (4B10B2) 1:1000 Sigma-Aldrich, SAB5300167
LC3 Rabbit polyclonal and Rabbit monoclonal (D11) to LC3B 1:1000 Cell Signaling Technology, 2775S and 3868S
HA Mouse monoclonal (16B12) to HA
Rabbit polyclonal to HA
1:1000
1:200 (microscopy)
1:100
Covance, MMS-101R
Abcam, ab137838
HIV Nef Mouse monoclonal (2H12) to HIV-1 Nef 1:1000 ThermoFisher Scientific, MA1-71505
HIV-1 Gag p55/p24 Mouse monoclonal (183-H12-5C) to HIV p24 1:1000
1:200 (microscopy)
NIH AIDS Reagent Program, 3537
HIV-1 gp120 Goat polyclonal to HIV-1 gp120 1:1000 Abcam, ab21179
HLA-ABC Mouse monoclonal (G46-2.6) to human HLA-ABC (APC-conjugated) 1:200 BD Biosciences 562006
SERINC5 Rabbit polyclonal to SERINC5 1:200 Sigma HPA037898
SQSTM1/p62 Mouse monoclonal to SQSTM1/p62 1:1000 Abcam, ab56416
SIV Nef Mouse monoclonal (17.2) to SIV Nef 1:1000 NIH AIDS Reagent Program, 2659
Mouse IgG Goat polyclonal (HRP-conjugated) 1:2000 Pierce, 31430
Mouse IgG1 Goat polyclonal (Alexa-568 conjugated) 1:500 ThermoFisher Scientific, A21134
Mouse IgG1 Goat polyclonal (Alexa-350 conjugated) 1:200 ThermoFisher Scientific, A21120
Rabbit IgG Goat polyclonal (HRP-conjugated) 1:2000 Abcam, ab97051
Rabbit IgG Donkey polyclonal (HRP-conjugated) 1:2000 Abcam, ab16284
Rabbit IgG1 Goat polyclonal (Alexa-488 conjugated) 1:200 ThermoFisher Scientific, A11008
Rabbit IgG Goat polyclonal (Alexa-568 conjugated) 1:200 ThermoFisher Scientific, A11011
Goat IgG Donkey polyclonal (HRP-conjugated) 1:2000 Abcam, ab6885