Table 1 Antibody sources and conditions

ACTB/β-actin

Mouse monoclonal (C4) to ACTB/β-actin

1:1000

Sigma-Aldrich, MAB1501

BCL2

Mouse monoclonal (124) to BCL2

1:1000

Cell Signaling Technology, 15071S

BECN1

Rabbit monoclonal (D40C5) to BECN1

1:1000

Cell Signaling Technology, 3495S

CD4

Mouse monoclonal (RPA-T4) to CD4 (FITC-conjugated)

1:200

BD biosciences, 561005

GFP

Mouse monoclonal (4B10B2)

1:1000

Sigma-Aldrich, SAB5300167

LC3

Rabbit polyclonal and Rabbit monoclonal (D11) to LC3B

1:1000

Cell Signaling Technology, 2775S and 3868S

HA

Mouse monoclonal (16B12) to HA

Rabbit polyclonal to HA

1:1000

1:200 (microscopy)

1:100

Covance, MMS-101R

Abcam, ab137838

HIV Nef

Mouse monoclonal (2H12) to HIV-1 Nef

1:1000

ThermoFisher Scientific, MA1-71505

HIV-1 Gag p55/p24

Mouse monoclonal (183-H12-5C) to HIV p24

1:1000

1:200 (microscopy)

NIH AIDS Reagent Program, 3537

HIV-1 gp120

Goat polyclonal to HIV-1 gp120

1:1000

Abcam, ab21179

HLA-ABC

Mouse monoclonal (G46-2.6) to human HLA-ABC (APC-conjugated)

1:200

BD Biosciences 562006

SERINC5

Rabbit polyclonal to SERINC5

1:200

Sigma HPA037898

SQSTM1/p62

Mouse monoclonal to SQSTM1/p62

1:1000

Abcam, ab56416

SIV Nef

Mouse monoclonal (17.2) to SIV Nef

1:1000

NIH AIDS Reagent Program, 2659

Mouse IgG

Goat polyclonal (HRP-conjugated)

1:2000

Pierce, 31430

Mouse IgG1

Goat polyclonal (Alexa-568 conjugated)

1:500

ThermoFisher Scientific, A21134

Mouse IgG1

Goat polyclonal (Alexa-350 conjugated)

1:200

ThermoFisher Scientific, A21120

Rabbit IgG

Goat polyclonal (HRP-conjugated)

1:2000

Abcam, ab97051

Rabbit IgG

Donkey polyclonal (HRP-conjugated)

1:2000

Abcam, ab16284

Rabbit IgG1

Goat polyclonal (Alexa-488 conjugated)

1:200

ThermoFisher Scientific, A11008

Rabbit IgG

Goat polyclonal (Alexa-568 conjugated)

1:200

ThermoFisher Scientific, A11011

Goat IgG

Donkey polyclonal (HRP-conjugated)

1:2000

Abcam, ab6885