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Fig. 7 | Retrovirology

Fig. 7

From: Residues T48 and A49 in HIV-1 NL4-3 Nef are responsible for the counteraction of autophagy initiation, which prevents the ubiquitin-dependent degradation of Gag through autophagosomes

Fig. 7

The ability of Nef to impair autophagy initiation is genetically separable from other functional roles of the protein. A, B HEK293T cells were co-transfected with SERINC5-HA and either an empty vector, NL4-3 nef or 48–49 NL4-3 nef. 48 h later cells were analyzed by flow cytometry to measure the surface levels of MHC-I (A), and SERINC5 relative to the empty vector control (B). C HeLa TZM-bl cells were transfected with an empty vector, NL4-3 nef or 48–49 NL4-3 nef. 48 h later, cells were analyzed by flow cytometry for their surface levels of CD4 relative to the empty vector control. Data correspond to the mean and SEM of three biological replicates. Histograms are representative of three independent experiments. Significantly different values are indicated by asterisks **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001

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Retrovirology

ISSN: 1742-4690

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