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Fig. 3 | Retrovirology

Fig. 3

From: Residues T48 and A49 in HIV-1 NL4-3 Nef are responsible for the counteraction of autophagy initiation, which prevents the ubiquitin-dependent degradation of Gag through autophagosomes

Fig. 3

Gag ubiquitination and membrane association are required to target Gag for autophagy elimination. A HEK293T cells were transfected with Gag, G2A-Gag, Ub-Gag or G2A/Ub-Gag. 48 h later, cells were harvested and LC3 immunoprecipitated. The pulldown fraction was examined for LC3, SQSTM1 and Gag. Lysates were also analyzed by western blot for the levels of SQSTM1, Gag, LC3 and ACTB. Bottom graph: Densitometric analyses to determine the relative LC3-Gag interaction. Data represent the mean and SEM of 3 independent replicates. B HEK293T cells were transfected with Gag, G2A-Gag, Ub-Gag or G2A/Ub-Gag and treated with rapamycin (4 μM) for 12 h. The cell lysates were analyzed by western blot for Gag, ACTB, and LC3. All images are representative of 3 independent experiments. Significantly different values are indicated by asterisks ***P ≤ 0.001

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Retrovirology

ISSN: 1742-4690

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