Table 5 Neutralization and binding properties of mAb 17b against WT and resistant Env
Neutralization IC50 (nM) (fold-change relative to WT) | ITCa | SPR | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
NL4-3 | HxBc2 | KD (nM) | ΔH (kcal/mol) | ΔG (kcal/mol) | −TΔS (kcal/mol) | N | KD (nM) | k1 (M−1 s−1) × 10–3 | k-1 (s−1) × 104 | |
Wild type | 1.3 ± 0.39 (1) | 6.1 ± 3.2 (1) | 280 ± 5.0 | − 33 ± 3.8 | − 9.0 ± 2.9 | 24 ± 6.7 | 1.0 ± 0.03 | 130 | 4.2 | 5.4 |
S143N | 2.0 ± 0.50 (1.6) | 6.2 ± 1.7 (1.0) | − | − | ||||||
V255I | 55 ± 29 (44) | 78 ± 42 (13) | 900 ± 20 | − 15 ± 2.6 | − 8.8 ± 1.6 | 6.4 ± 4.2 | 1.5 ± 0.09 | 500 | 1.5 | 7.4 |
V255T | 110 ± 26 (89) | 120 ± 100 (19) | – | – | ||||||
V255I/S143N | 250 ± 190 (200) | 250 ± 110 (40) | 1000 ± 20 | − 17 ± 1.6 | − 7.6 ± 1.2 | 9.3 ± 2.8 | 1.1 ± 0.06 | 530 | 1.8 | 9.4 |
V255T/S143N | 270 ± 89 (220) | 350 ± 85 (57) | – | – | ||||||