Fig. 9

Impact of PT escape mutations on CD4- and AAR029b-induced gp120 shedding. A The percent of intact Env trimers remaining on HEK293T cells expressing wild type and resistant NL4-3 Env exposed to serial dilutions of sCD4 (5000 nM as the highest concentration) at 37 °C for 2 h. The measurements (mean fluorescence intensity) were obtained by flow cytometry using an anti-Env IgG (mAb 35O22) that recognizes a quaternary epitope at the gp120/gp41 interface. B As in A, except that the cells were exposed to serial dilutions of AAR029b (1 μM as the highest concentration). Data represent the mean and standard error of four independent experiments performed in quadruplicate