Fig. 8

Effect of PT escape mutations on CD4 binding and utilization during entry. A Binding paramerters derived from the interaction of sCD4 with WT, V255I and V255I/S143N monomeric gp120 variants as measured by surface plasmon resonance (sensorgrams shown in Additional file 1: Figure S3). B Infectivity of HIV-1 pseudotyped with wild type and resistant Env variants in the absence and presence of CD4 antagonist D23.2 (11 nM). The significance of data was determined by two-way ANOVA in GraphPad Prism 9 (*p < 0.05, ***p < 0.0005, ****p < 0.0001). C Relative infectivities (normalized data from B) to illustrate the impact of escape mutations on D23.2 sensitivity. Data represent the mean and standard error of three independent experiments done in quadruplicate. There was no significant difference between groups as assessed by one-way ANOVA in GraphPad Prism 9