Fig. 5

Kinetic analysis of AAR029b and KR13 binding to wild type and resistant gp120 by surface plasmon resonance. The Biacore 3000 sensorgrams depict the interaction of AAR029b (A–C) and KR13 (D–F) with wild type (A and D), V255I (B and E) and V255I/S143N (C and F) HxBc2 gp120 variants covalently attached to the sensor chip surface at 25 °C. The global fits to two replicates, as defined in Materials and Methods, are shown as red lines. The binding parameters derived are summarized in Table 4