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Fig. 1 | Retrovirology

Fig. 1

From: Arginyl-tRNA-protein transferase 1 contributes to governing optimal stability of the human immunodeficiency virus type 1 core

Fig. 1

ATE1 interacts with HIV-1 Pr55gag and CA-NTD. A Bait constructs obtained from pNL-CH are illustrated. B Y2H analysis of ATE1 with CA, Pr55gag, CA-NTD or CA-CTD. The Y2HGold strain was cotransformed with ATE1 as prey protein and viral proteins as bait proteins. Growth on QDO/X/A plates with blue colonies indicates a positive interaction. Shown are data from three experiments with a similar outcome. C Determination of ATE1-Pr55gag and p160gag − pol interaction. ATE1 was immunoprecipitated from the clarified lysate from CEM/LAV-1 cells with anti-ATE1 antibody and co-immunoprecipitated proteins were detected using anti-p24 antibody

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