Fig. 4
From: An emerging and variant viral promoter of HIV-1 subtype C exhibits low-level gene expression noise
C-RRE exhibits low level gene expression. A Longitudinal gene expression analysis. RN3-LTR CEM-CCR5LR cells containing B-RRE or C-RRE were infected with NL4-3_ΔEnv_EGFP or Indie-C1_ΔEnv_d2EGFP molecular clones as a source of Tat and Rev. The expression of the fluorescence proteins was monitored over one week by flow cytometry. The data are representative of three independent experiments. B The total percentage of RFP+ve cells and (C) Non-responding RFP−ve cells are plotted. Two-way ANOVA with Tukey post-test was used to compare the data. D Droplet digital PCR was used to quantify the proviral copy number of Indie-C1_ΔEnv_d2EGFP infection. A two-tailed unpaired t-test (p = 0.14) was used to compare the data