Fig. 3
From: An emerging and variant viral promoter of HIV-1 subtype C exhibits low-level gene expression noise
The genetic diversity of RRE may modulate gene expression (A) TNF-α activation of cells. CEM-CCR5LR cells harboring RN3 reporter viral vector containing B- or C-RRE element were activated with PMA (5 ng/ml), and the levels of RFP were measured at 24 h by flow cytometry. An unpaired two-tailed t-test was performed to compare the data. B Viral infection of reporter cells. CEM-CCR5LR cells harboring RN3 reporter viral vector containing B- or C-RRE element were independently infected with one of four viral strains representing different subtypes—Q23-17 (HIV-1A), NL4-3 (HIV-1B), Indie-C1 (HIV-1C), or 94UG114.1.6 (HIV-1D). RFP expression was monitored as described above. An unpaired two-tailed t-test was performed to compare the data