Fig. 2

The transactivation profiles of the variant LTRs (A) RFP expression and (B) luciferase expression. CEM-CCR5_LR reporter cells were activated with TNF-α (10 ng/ml), PMA (5 ng/ml), or with a cocktail of four activators (10 ng/ml TNF-α, 5 ng/ml PMA, 100 nM TSA, and 2.5 mM HMBA). The expression levels of RFP were measured 24 h following activation. Each data point represents the mean of three replicate values. The data are presented as the mean percentage value ± SD of RFP positive cells from three independent experiments. We used ordinary one-way ANOVA with Sidak’s multiple comparison test (ns no significant, *p < 0.05, ***p < 0.05, ****p < 0.0001) for statistical analysis. C Transactivation profile following viral infection: cells were infected with NL4-3 viral strains as a source of Tat and Rev and the RFP expression levels were quantitated as described above. One-way ANOVA with Sidak’s multiple comparison test was used (ns no significant and ***p  < 0.05)