Fig. 5
From: Derivation and characterization of an HIV-1 mutant that rescues IP6 binding deficiency
Visualization of Bevirimat-induced assembly by fluorescence microscopy. A Fluorescence microscopy of TZM cells infected with a reporter HIV-1 virus encoding mNeon Green in place of Pol (HIV-1 NG) to visualize assembly. Representative images 48 h-post-infection of HIV-1 NGWT, HIV-1 NGK359A, or HIV-1 NGP289S infected in the absence or presence of 5 μM BVM. Quantification of Gag-NG punctae per image depicts mean ± SD for images from (A). B Representative time lapse images of TZM cells infected with HIV-1 NGP289S −/+ treatment with 5 μM BVM at 26 h post infection. Image acquisition began immediately after BVM addition. Image labels: Hours:Minutes:Seconds post BVM addition. Quantification of Gag-NG punctae per timepoint depicts mean ± SD for images from B. C Representative time-lapse images of TZM cells treated with BVM at 26 h post infection with HIV-1 NGP289S. Image labels: Hours:Minutes:Seconds post BVM addition. Quantification of Gag-NG punctae per timepoint depicts mean ± SD for images from C