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Fig. 3 | Retrovirology

Fig. 3

From: Derivation and characterization of an HIV-1 mutant that rescues IP6 binding deficiency

Fig. 3

Bevirimat rescues infectivity of HIV-1K359A. A 293T cells were transfected with HIV-1WT, HIV-1K359A, or HIV-1P289S proviral plasmids in the presence of 0, 1, 5, or 10 μM Bevirimat. After 48 h, supernatants were collected and titrated on MT4 cells and infection quantified by flow cytometry. B, C MT4 cells (B) or CEM cells (C) were infected with HIV-1WT, HIV-1K359A, or HIV-1P289S at an MOI of 0.001. At 16 h post infection, inoculum was washed away and supernatants were collected at the indicated timepoints. Reverse transcriptase activity was measured in the supernatant samples using SYBR-PERT

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