Fig. 2
From: Derivation and characterization of an HIV-1 mutant that rescues IP6 binding deficiency
HIV-1K359A/T371I is not impaired by lack of cellular IP6. A–D Control or IPMK KO single 293T cell clones were transfected with HIV-1WT (A), HIV-1K359A (B), HIV-1T371I (C), or HIV-1K359A/T371I (D) proviral plasmids. After 48 h, supernatants were collected and titrated on MT4 cells. Each data point represent a different 293T cell clone. Statistical analysis: unpaired Student’s t-test. Alternatively, levels of Reverse Transcriptase in supernatants were quantified using the SYBR-PERT assay (E–H). I, J HIV-1WT (I) or HIV-1K359A/T371I (J) virions were titrated on WT MT4 or IPMK KO MT4 cells and infection quantified by flow cytometry. Each data point represent a different MT4 cell clone. Statistical analysis: unpaired Student’s t-test