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Fig. 2 | Retrovirology

Fig. 2

From: Derivation and characterization of an HIV-1 mutant that rescues IP6 binding deficiency

Fig. 2

HIV-1K359A/T371I is not impaired by lack of cellular IP6. AD Control or IPMK KO single 293T cell clones were transfected with HIV-1WT (A), HIV-1K359A (B), HIV-1T371I (C), or HIV-1K359A/T371I (D) proviral plasmids. After 48 h, supernatants were collected and titrated on MT4 cells. Each data point represent a different 293T cell clone. Statistical analysis: unpaired Student’s t-test. Alternatively, levels of Reverse Transcriptase in supernatants were quantified using the SYBR-PERT assay (EH). I, J HIV-1WT (I) or HIV-1K359A/T371I (J) virions were titrated on WT MT4 or IPMK KO MT4 cells and infection quantified by flow cytometry. Each data point represent a different MT4 cell clone. Statistical analysis: unpaired Student’s t-test

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