Fig. 7

ADCC activity against HIV-1-infected cells mediated by combination of anti-C1C2 antibodies, an anti-CoRBS antibody and CD4mc. The ability of 1E5 to mediate ADCC activity was analyzed by measuring the decrease of HIV-1-infected cells. NKR24 cells infected with HIV-1, pNL-AG-257.ecto and pNL-AG-280.ecto, and N6 cells were used as target and effector cells, respectively. Decrease of HIV-1-infected cells was calculated from luciferase activity, which was increased by the LTR-driven luciferase gene in HIV-1-infected CEM.NKR-CCR5 cells. Antibodies, 1E5-IgG3 (5 µg/ml), A32 (1.25 µg/ml) and 4E9C (5 µg/ml) were used solely or in combination in the presence or absence of YIR-821 (20 µM)