Fig. 4

Anti-CoRBS antibody and CD4mc increased binding of 1E5 to CRF02_AG Env. HEK 293T cells were transfected with plasmid expressing both EGFP and CRF02_AG-257 Env. At 48 h post-transfection, cells were stained with biotinylated test IgG (1E5-IgG1 and 1E5-IgG3, 10 µg/ml) alone or with anti-CoRBS antibody (17B or 4E9C, 5 µg/ml) in the presence or absence of CD4mc YIR-821 (20 µM). Biotinylated A32 and biotinylated NHG were used as a control. Then, cells were fluorescently labeled with APC-conjugated streptavidin. Histograms of APC in EGFP⁺ cells are shown; Biotinylated NHG (gray shading), test IgG alone (orange), test IgG and YIR-821 (green), test IgG and anti-CoRBS antibody (blue), and IgG, YIR-821 and anti-CoRBS antibody (red). The representative result from three independent experiments is shown. Each independent experiment was done at once with the same transfected cells