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Fig. 1 | Retrovirology

Fig. 1

From: Advancing our understanding of HIV co-infections and neurological disease using the humanized mouse

Fig. 1

HIV Infection of Multiple Tissue Compartments Supports Potential for Co-infection Investigations in HIS Mice, including in CNS. Results shown in AD are from mice that were infected with 2500 TCID50 of HIV JR-CSF using an i.v. route and tissue collected after 3 weeks of infection. Autopsy specimens of human brain displaying HIV encephalitis are shown in E and F Formalin-fixed and paraffin embedded tissue sections were cut, dewaxed, and viral RNA (AE) or DNA (F) detected by using in situ hybridization with probes specific to the HIV-1 gag gene using RNA Scope® or DNA Scope® (ACD Bio) (AF). Staining was visualized with bright field microscopy. Representative images of results from mice used in various studies is shown. Results displayed in GI are from HIS BLT mice infected i.n. with 102 CFU of Mtb H37Rv for 4 weeks. Shown is development of TB encephalitis as visualized with H&E (G) and pockets of AFB (H) in the inflammatory lesion. Brains of Mtb-infected mice lacking significant neuropathology (I) also occasionally presented with a rare AFB (I). These results are illustrative of observations from several different studies performed with humanized NSG-BLT mice with mono-infections of HIV or Mtb

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