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Fig. 1 | Retrovirology

Fig. 1

From: Neutralization diversity of HIV-1 Indian subtype C envelopes obtained from cross sectional and followed up individuals against broadly neutralizing monoclonal antibodies having distinct gp120 specificities

Fig. 1

Association of phylogenetic relatedness of env and their responses when expressed as pseudoviruses to the four bnAbs: a A phylogenetic tree was constructed for gp160 amino acid sequences of viral clones reported in the present study along with those reported in the CATNAP database (Total N = 1020). The terminal branches of the tree were color-coded based on the subtype as depicted in the color legend. Four heatmaps based on their responses to bnAbs PGDM1400, PGT121, VRC01 and CAP256.-VRC26.25 were overlayed on the phylogenetic tree in the form of concentric tracks one for each bnAb to assess phylogenetic clustering of their IC50 values. IC50 value of 5 µg/mL were considered as neutralization sensitivity threshold. b Neutralization potency (scatter plot against left Y axis) was plotted for pseudoviruses expressing 98 subtype C envs (71 from the present study) from India against the four bnAbs. Pink lines indicate median IC50 values. Orange dots represent data generated in the present study while the black dots indicate data retrieved from the CATNAP database. Potency against all the pairs of bnAbs were compared using Mann–Whitney test. p values were illustrated as follows: > 0.05-non-significant, 0.05–0.01- *, 0.01–0.001-** and < 0.001-***. Percent neutralization coverage of all the bnAbs were plotted as a bar chart against the right Y axis. c Year matched randomly selected equal number viral clone datasets were retrieved from CATNAP database and their IC50 values to 4 bnAbs (PGT121, PGDM1400, VRC01 and CAP256-VRC26.25 were compared between Subtype C from India, Subtype C pan Africa and Other subtypes (All except C). IC50 value of 5 µg/mL was considered as a threshold of neutralization sensitivity. Statistical comparisons were made between each pair for every antibody using Mann–Whitney test

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