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Fig. 8 | Retrovirology

Fig. 8

From: A single G10T polymorphism in HIV-1 subtype C Gag-SP1 regulates sensitivity to maturation inhibitors

Fig. 8

Mutation in HIV-1 subtype C K3016 Gag-SP1 residue 10 decreases sensitivity towards BVM analogs. a HEK-293 T cells were transfected with the HIV-1 subtype C clones, K3016 WT and its mutants SP1: N9S, SP1:G10T and SP1:N9S/G10T. Cells were treated with 100 nM of BVM analogs CV-8611, CV-8612, CV-8613 or with DMSO only. The virion-associated CA and CA-SP1 were detected by immunoblotting. Quantification of % CA-SP1 relative to total CA + CA-SP1 is presented in the graphs. The gel image shown here is representative of three independent experiments. b TZM-bl cells were infected with p24 normalized HIV-1 subtype C viruses K3016 WT and its mutants, SP1:N9S, SP1:G10T and SP1:N9S/G10T produced from MI treated HEK-293T cells for 48 h to measure infectivity. Quantitative data for levels of infectivity relative to the DMSO control-treated sample is shown. Error bars indicate standard deviations from three independent experiments. The asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and ns indicates not significant

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