Fig. 3

BVM analogs delays HIV-1 subtype C K3016 replication. The HUT-R5 T-cells were transfected with the K3016 molecular clone. Transfected cells were proliferated in the presence of 10 nM and 100 nM of a BVM analog CV-8611 or with DMSO only, b BVM analog CV-8612 or with DMSO only and c BVM analog CV-8613 or with DMSO only. Virus replication was observed by quantifying HIV-1 p24 concentration in the culture supernatant, and the replication profile was represented as a graph. d The HUT-R5 T-cells were transfected with K3016 molecular clone and propagated in the presence of 100 nM of the BVM analogs (CV-8611, CV-8612, and CV-8613). The virus produced was re-passaged multiple times in HUT-R5 T-cells. The Gag and Protease coding region of the HIV-1 virus was sequenced to analyze the resistance profile. The figure depicts the sites of the mutation