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Fig. 2 | Retrovirology

Fig. 2

From: Extracellular vesicles from HTLV-1 infected cells modulate target cells and viral spread

Fig. 2

Proteomic analysis of Human proteins in HTLV-1 EVs. a Venn diagram of 2 k, 10 k, and 100 k EVs showing total number of proteins identified (in parenthesis) and unique proteins (underneath total proteins). Mass spectrometry data was searched against fully tryptic indexed Homo sapiens database (UniProt) via Proteome Discoverer 2.1. Next, Protein–Protein Interaction (PPI) network was evaluated for unique proteins in b 2 k, c 10 k, and d 100 k EVs. Predicted PPIs were analyzed by STRING to determine the pathway interaction of proteins in each EV. PPIs were analyzed from STRING to determine the pathway enriched in each EV population. In the PPI network, nodes represent Proteins (circles), Edges represent Protein–Protein Associations (lines), and color represent related pathway or family of protein (colored circles). High confidence levels of 0.7–0.9 were used for this analysis. e HTLV-1 EVs (2 k, 10 k, and 100 k) and f control EVs (CEM and U937) were evaluated using a Luminex platform for presence of encapsulated or surface-bound cytokines. Only 9 representative EVs were selected out of a panel of 33 cytokines. Red boxes show the most abundant cytokines across the different EV populations

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