Fig. 1

Tax and p19 viral proteins are present in EV populations derived from HTLV-1 infected cells. a Viral protein distribution was evaluated in different EV populations (i.e., 2 k, 10 k, and 100 k) derived from HUT102 cells. Mass spectrometry data was searched against a fully tryptic HTLV-1 database (UniProt) via Proteome Discoverer 2.1. b Different EV populations (2 k, 10 k, and 100 k) of HUT102 were obtained via differential ultracentrifugation and 30 μg was loaded into each lane. Viral proteins (p19 and Tax), EV marker (CD63), autophagy proteins (LC3-I, LC3-II, and p62) and Actin were detected using Western blot. c Quantification of Western blot bands by densitometry analysis