Fig. 5
From: Vpx enhances innate immune responses independently of SAMHD1 during HIV-1 infection
a–c THP-1 Lucia ISG cells (undifferentiated) were infected with HIV-1GFP in the presence or absence of VLPs containing WT or Q76A Vpx. a Infected cells were analyzed by flow cytometry 2 days post-infection. b ISRE reporter induction was assessed by lucia luciferase assay of supernatants from infected cells. c Cell lysates were collected from infected cells and analyzed on a Western blot, probing for SAMHD1. d–f THP-1 Lucia ISG cells (undifferentiated) were infected with a single-round HIV-2GFP with or without its own Vpx. d Virions were run on a Western blot and probed for p26 CA using a p24 antibody known to cross react with HIV-2 CA [47]. Infection was quantified by flow cytometry (e) and ISRE induction was measured by luciferase activity (f). *p < 0.05, **p < 0.01