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Fig. 4 | Retrovirology

Fig. 4

From: Vpx enhances innate immune responses independently of SAMHD1 during HIV-1 infection

Fig. 4

SAMHD1 KO THP-1 cells (undifferentiated) were infected with HIV-1GFP in the presence or absence of VLPs containing Vpx. a Lysates from WT and KO cells were analyzed for SAMHD1 by Western blot. b Differences in basal mRNA levels between WT and KO cells for a panel of ISGs. c Infection levels were analyzed by flow cytometry. d Supernatants from infected cells were incubated with HEK-Blue IFN-α/β cells (HEK-Blue); SEAP assay was performed the next day to assess the presence of type I IFN in culture supernatants of infected cells. e RNA was isolated from infected cells and qRT-PCR was performed to quantify the mRNA levels of IFNB1, IFIT1 and IFIT2 (relative to HPRT1), and normalized to uninfected cells. f SAMHD1 KO THP-1 cells (undifferentiated) were infected HIV-1GFP with or without virion packaged Vpx, in the presence and absence of 10 µM NVP. Supernatants were analyzed for IFN-β secretion by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant

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