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Fig. 3 | Retrovirology

Fig. 3

From: Vpx enhances innate immune responses independently of SAMHD1 during HIV-1 infection

Fig. 3

a THP-1 Lucia ISG cells (undifferentiated) were infected with serial dilutions of VSV-G pseudotyped HIV-1GFP reporter viruses; nevirapine (NVP; 10 µM) was added as a control. ISRE reporter was quantified by lucia luciferase expression 2 days later. b Cells infected at the highest dose were analyzed by flow cytometry showing the shift in FSC/SSC profiles. c Live/dead staining vs. GFP expression confirmed cell death. df cells were challenged with HIV-1GFP in the presence or absence of NVP (10 µM) or with heat inactivated virus. Infection efficiency (d) and cell death (e) were evaluated by flow cytometry, ISRE reporter activity was quantified by luciferase assay of culture supernatants (f)

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