Fig. 2
From: Vpx enhances innate immune responses independently of SAMHD1 during HIV-1 infection
THP-1 Lucia ISG cells (undifferentiated) were infected with serial dilutions of VSV-G pseudotyped HIV-1Luc reporter viruses with or without packaged SIVmac Vpx. a Infection was quantified by firefly luciferase expression 2 days later. b ISRE reporter was quantified by lucia luciferase expression. c Comparison of infection vs. ISRE levels. d IFN-β was titrated on undifferentiated THP-1 reporter cells and luciferase activity in culture supernatants was measured. e Total cell lysates were analyzed by Western blot and probed with the indicated antibodies. f Representative Western blot of undifferentiated and PMA-differentiated cells (days 1 and 4 after PMA-treatment), showing phospho-T592 and total SAMHD1 levels. Data are from three replicates; error bars depict standard deviation