Fig. 3

Immune activation and latency reversal after stimulation of CD8 depleted PBMC with antigen peptide pools measured by droplet digital (dd)PCR of cellular Tat/Rev mRNA. a Flow cytometric detection of CD69 expression levels on CD4 T cells after 48 hours stimulation with the indicated peptide pools. All peptide pools were used at a concentration of 1 µg/ml per peptide. b Expression levels of cell-associated multiply spliced HIV-1 Tat/Rev after five days stimulation with the indicated peptide pools (n = 6). Nine-million cells from each participant were stimulated as indicated, then plated in three-fold limiting dilutions and six replicates. “ELISpot” refers to a custom, participant-specific Gag-peptide pool selected by IFN-γ ELISpot assay of individual peptides. Cells were incubated in the presence of raltegravir to prevent viral propagation. Each participant’s cells are shown using a distinct symbol. Some participant’s cells were not tested with every peptide pool. Values were normalized to the positive control (antibodies to CD3 and CD69) for each participant. DMSO is the negative control