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Fig. 1 | Retrovirology

Fig. 1

From: Inconsistent reversal of HIV-1 latency ex vivo by antigens of HIV-1, CMV, and other infectious agents

Fig. 1

IFN-γ ELISpot. a CD4 T cell response to pooled 15mer peptides with 11 amino acid overlap, specific for: CMV pp65 (138 peptides), CEFT (14 peptides selected from defined HLA class-II restricted T-cell epitopes of Clostridium tetani, Epstein-Barr virus (HHV-4), human cytomegalovirus (CMV; HHV-5), and Influenza A), HIV-1 consensus B Gag (123 peptides), HIV-1 consensus B Env (211 peptides), HIV-1 consensus B Pol (249 peptides), and HIV-1 consensus B Nef (49 peptides). Example wells are shown for each peptide pool; the examples are from different participants. Below are the bar graphs of three study participants depicting the IFN-γ response to the tested peptide pools. b CD4 T cell responses of three participants towards 123 individual overlapping 15mer HIV-1 consensus B Gag peptides. The x-axis shows the sequence of every other single peptide. SFC spot forming cells. Marked by asterisks are the respective peptides that were subsequently used for the participants’ customized ELISpot-selected Gag peptide pools. c Comparison of PBMC (bottom) and CD8 depleted PBMC from the same blood donor. Marked by asterisks are peptides that were associated with CD8 T cell responses that were not present in the assay using CD8 depleted PBMC

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