Fig. 5

ENO1 in viral producer and viral target cells has bifunctional inhibitory activities against HIV-1 replication. a Infection assay to confirm bifunctional inhibitory activity of ENO1. A high-level-ENO1-packaging virus derived from pNL-CH-transfected HEK293 cells and ENO1-overexpressing TZM-bl cells were prepared with an ENO1-V5 expression vector. The bifunctional inhibitory activity of ENO1 was examined by infecting target cells with different ENO1 expression levels using viruses with different amounts of ENO1 incorporated into their particles. b Infection assay to confirm bifunctional inhibitory activity of ENO1. A low-level-ENO1-packaging virus derived from CEM/LAV-1 cells treated with ENO1-specific siRNA and ENO1-overexpressing TZM-bl cells were prepared. The value in the control experiment (target cells and producer cells are shown as normal or WT) was set as 100%. Data are mean values ± SE from triplicate tests. The significance of difference (Nonrepeated measures ANOVA and Dunnett’s test versus control) is indicated as follows: **, p < 0.01; n.s., not significant