Fig. 4
From: Alpha-enolase in viral target cells suppresses the human immunodeficiency virus type 1 integration
ENO1 is present in the nucleus of the target cell. a Observation of endogenous ENO1 and ENO1-V5 by fluorescence microscopy. Endogenous ENO1 (left top panel) and ENO1-V5 (right top panel) were visualized by staining with the anti-ENO1 antibody and anti-V5 antibody, respectively. Nuclei were visualized by DAPI staining (middle column). The ENO1 or ENO1-V5 signal and DAPI signal are shown as merged (right column). In the right lower panels, cells transfected with the control vector were also analyzed. Small amounts of ENO1 and ENO1-V5 colocalized in the nucleus. A representative image from three independent experiments is shown. Subcellular fractionation of b TZM-bl cells, c CEM cells and d HIV-1LAV-1-infected TZM-bl cells. b–d Endogenous ENO1 was detected by staining with the anti-ENO1 antibody and ENO1-V5 was detected by staining with the anti-V5 antibody. LDH and HDAC1 were detected as the cytosolic and nuclear markers, respectively. Fractionation was performed from the same cell number and then the amount loaded to each lane was calculated by the BCA method