Fig. 1
From: Alpha-enolase in viral target cells suppresses the human immunodeficiency virus type 1 integration
ENO1, but not GAPDH, in target cells affects HIV-1 replication. a Western immunoblotting monitoring of GAPDH expression in either CEM cells or CEM/LAV-1 cells. b Confirmation of knockdown efficiency of GAPDH-specific siRNA in TZM-bl cells. c HIV-1LAV-1 infectivity in either control or GAPDH-knockdown TZM-bl cells. The value in the control-siRNA-treated TZM-bl cells was set as 100%. d Western immunoblotting monitoring of ENO1 expression in either CEM cells or CEM/LAV-1 cells. e Confirmation of knockdown efficiency of ENO1-specific siRNA in TZM-bl cells. f HIV-1LAV-1 infectivity in ENO1-knockdown TZM-bl cells. The infectivity in the control-siRNA-treated TZM-bl cells was set as 100%. g Effects of ENO1 siRNA treatment on viability of TZM-bl cells. Viable cells were evaluated by trypan blue staining. The infectivity was examined at 2 days postinfection with HIV-1LAV-1 and assessed on the basis of the luciferase activity in lysates of any siRNA-treated TZM-bl cells. Data are mean values ± SE from triplicate tests. The significance of difference (Student’s t-test) is indicated as follows: **, p < 0.01; n.s., not significant