Table 2 Properties determined by sedimentation equilibrium analysis
From: Influence of the amino-terminal sequence on the structure and function of HIV integrase
Protein | Concentrations (µM) | Speeds (krpm) | Model fit | Massa (Da) | Kd (µM) | Global reduced χ2 |
|---|---|---|---|---|---|---|
IN 1F | 8.9, 14.5 | 16,20,24 | M–D | 32,330 | 59.8 ± 6 | 2.7 |
IN MF | 6.0, 7.9 | 16,20,24 | M–D | 32,199 | 126.7 ± 23 | 1.3 |