Fig. 5
From: Influence of the amino-terminal sequence on the structure and function of HIV integrase
Biophysical analysis of IN 1F and IN MF. a SEC-MALS analysis of IN 1F and IN MF. Both the Mw (weight-average molecular mass) from multiangle light scattering and retention times are consistent with mixtures of monomers and dimers for both IN 1F and IN MF (expected MW of monomer: 32 kDa). b Sedimentation velocity analysis of IN 1F and IN MF shows distinct populations of monomer and dimer in solution. c(S) distributions derived from the fitting of the Lamm equation are shown. c Sedimentation equilibrium analysis of IN 1F and IN MF indicates the presence of monomers and dimers in solution at 4 °C. Globally fit radial distributions for 8.9 μM (IN 1F) and 6 μM (IN MF) in a monomer–dimer model are shown. Table 2 provides the association properties derived from this analysis