Fig. 4

ALLINI-induced aggregation of IN 1F, IN GSH, and IN MF. a Aggregation of IN at 15 µM was induced by incubation with 30 µM of the ALLINIs BI-224436, BI-D, or CX04328 at a range of NaCl concentrations for 20 min. Aggregation was measured by light scattering at 405 nm. No aggregation was observed for any ALLINI at 1000 mM NaCl. At intermediate NaCl concentrations, ALLINIs induce aggregation of IN 1F more potently than IN GSH or IN MF. b Aggregation in the absence of ALLINIs. No aggregation was observed at NaCl concentrations of 300 mM or greater. Below 300 mM, IN 1F, and, to a lesser extent, IN MF and IN GSH spontaneously aggregate. * Denotes P < 0.05, ** denotes P < 0.01, *** denotes P < 0.001, **** denotes P < 0.0001