Fig. 2

3′-processing activity in vitro. a Diagram of 3′-processing fluorescence polarization assay. The double stranded oligo containing a 3′ fluorescent label exhibits high fluorescence polarization. Cleavage and release of the terminal dinucleotide causes a decrease in fluorescence polarization. 5′-ends are designated by filled circles and the fluorophore is designated by the green star. b 3′-processing activity of IN 1F compared to IN GSH (left) and IN 1F compared to IN MF (right) in the presence of Mg²⁺. c 3′ processing activity of IN 1F compared to IN GSH (left) and IN 1F compared to IN MF (right) in the presence of Mn²⁺. Data are plotted as mean ± SD. ** Denotes P < 0.01 and **** denotes P < 0.0001