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Fig. 6 | Retrovirology

Fig. 6

From: Dynamic nanopore long-read sequencing analysis of HIV-1 splicing events during the early steps of infection

Fig. 6

Relative abundance of viral transcripts expressed at early time points of T cell infection, determined by ONT sequencing. Primary CD4+ T cells were infected with HIV NL4-3 VSV-G pseudotyped virus and harvested at 12, 14, 16, 20 and 24 hpi for RNA extraction. Relative abundance of a Total, b MS, c Env/Vpu 1 and d US RNA was monitored by qPCR for three different donors using the ΔΔCq method and normalized with GAPDH and β-Actin as reference genes. ONT sequencing and mapping were performed on RNA extracted from donor 4 (orange lines). Read counts were normalized with DESeq 2 included in the Eoulsan’s pipeline. Abundance of viral RNA classes was calculated as in Fig. 3. All values were expressed as fold enrichment over the 12 h point. e Relative levels of the most abundant viral isoforms detected between 12 and 24 hpi were calculated as in Fig. 3, normalized using DESeq 2 and expressed as fold enrichment over the 12 h point

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